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Title: Efeitos do tabagismo durante a gestação em sangue e cordão umbilical de neonatos
Authors: Rua, Ednildes de Almeida Olympio
metadata.dc.contributor: Pereira, Thiago de Melo Costa
Keywords: Estresse oxidativo - Apoptose - Citometria de fluxo - AOPP - Fumantes
Issue Date: 27-May-2014
Abstract: Smoking is still a major health problem in Brazil and the world, one preventable cause of poor obstetric outcome. Although cigarette smoking is known to be a complex mixture of over 4000 substances that damage can lead to active or passive smoking.Its mechanisms and biochemical consequences are not yet fully understood, especially in pregnant women and neonates. Therefore, we propose a new way, through the use of flow cytometry to study the impact of smoking during pregnancy on the viability of blood mononuclear cells from umbilical cords of newborns in order to verify the degree of oxidative stress and cell viability and investigate possible biochemical and morphological changes in the umbilical cord. To this end, cord blood was collected and the umbilical cord itself hospitalized postpartum women in public hospitals in Greater Victoria during the third stage of labor, birth and after the cord clamping, and after detachment of the placenta and sterilization of a segment of cord. Then the cord blood was subjected to flow cytometry assays and the umbilical cord for biochemical and histological tests. Data are expressed as mean ± SEM and statistical analysis were performed Student t test (* p <0, 05). In the group of patients analyzed there was no difference between parameters such as age, blood pressure or gestational average and hematimetric indices. In neonates, parameters such as weight, Capurro index, head circumference and Apgar scores also showed no difference. However, analysis by flow cytometry showed that smoking during pregnancy harms the quality of blood mononuclear cells from umbilical cord : there was a 10% decrease in cell viability , increase in reactive oxygen species in relation to pregnant women not exposed (DHE : 1887 ± 189 * ; DCF 814 ± 66 vs au * DHE : 987 ± 71 DCF : 532 ± 27 au , respectively) and an increase of approximately 2 fold the rate of apoptosis ( 38 ± 4.5 % * vs. 19 ± 3.7 % . , respectively). Cord blood was demonstrated increased protein oxidation (232.1 ± 52.9 vs. 94.3 ± 13.9, mol / mg protein, respectivly) and loss of endothelial integrity by scanning electron microscopy, no changes of remodeling in both arteries and veins in the cord, as assessed by morphometric studies. Therefore, our data show that exposure to smoking can impair the viability of mononuclear cells derived from the umbilical cord and the very integrity of the umbilical cords of women exposed to cigarette smoke. These results by flow cytometry provide new directions of investigations of the impact of smoking on neonates.
Appears in Collections:Ciências Farmacêuticas (Dissertações)

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