Levantamento de microrganismos no ambiente do Biotério da Universidade de Vila Velha

Abstract

The study conducted aimed to assess the presence of microorganisms in the bioterium environment at the University of Vila Velha, focusing on the practices followed in the areas where rats and mice are bred and housed for scientific research. The research involved two rooms: one for rats and another for mice, with samples collected using sterilized swabs from the walls, each covering a 50 cm² area. Peptone water (0.1%) was used as a diluent for extracting the samples. Blood agar was employed for bacterial culture growth, alongside tests like catalase, coagulase, and Gram staining for microorganism identification. The research also involved microscopic analysis to monitor microbial growth and the results of these tests over several weeks. The study successfully identified the growth of Staphylococcus bacteria, confirming the presence of microorganisms and suggesting areas for improvement in hygiene practices. These findings highlight the need for further actions to ensure the animals’ welfare and the integrity of scientific results in the bioterium.