Efeitos de doses terapêuticas de dimetilsulfóxido (DMSO) sobre asma experimental induzida via câmera de nebulização

Abstract

Asthma is a chronic inflammatory disease of the airways, with high prevalence and morbidity. In veterinary medicine, the species that present asthmatic symptoms most frequently are felines and equines. Studies report that air pollution directly affects asthmatic symptoms: its effects are elucidated through experimental studies using animal models, especially rodents. To investigate asthmatic symptoms, induction protocols in mice via inhalation chambers have proven to be less invasive and closer to the reality of exposure to the allergen. Dimethyl sulfoxide (DMSO) is an organic substance widely used in laboratory and clinical applications, and the objective and scientific interest of this study is to verify its immunomodulatory effect on asthma. Mice were divided into three experimental groups: control group, immunized and untreated group, and immunized and treated with DMSO group. The animals underwent a challenge and were euthanized for collection of blood and lymph node samples, analyzed by flow cytometry. The use of therapeutic doses of DMSO in asthma-induced mice resulted in a proportional decrease in CD4 T cells in the mesenteric lymph node, demonstrating that DMSO acted on immunological orchestrators of asthma.