Efeitos de suplementos orais não-herbáceos e herbáceos em camundongos

Abstract

Objective: Recent findings in humans suggest that supplementation with non herbal and herbal products may be harmful to health. Therefore, the aim of this study was to evaluate the effect of chronic use of two oral supplements (Shake and Tea) in Balb/C mice. Methods: For this purpose, 46 male mice were divided into 4 groups: 1) “Control” (semi skimmed milk,0.5mL/day,n=10); 2) “Shake” (0.5mL/day,n=12); 3) “Tea” (0.5mL/day,n=12); 4) “Shake+Tea” (0.25mL/day each,n=12) for 28 days. Mice and feed were weighed daily, and glycemia of animals and the motor behavior were monitored weekly. After euthanasia, serum biochemical analysis was performed followed by hepatic and renal histological analysis. Moreover, the oxidative stress was determined through advanced oxidation protein products (AOPP) analysis. In relation to chemical analysis, quantification of caffeine in supplements was performed by thin layer chromatography and high performance liquid chromatography. Results: Although the chronic use of non herbal and herbal products may be able to contribute to weight control, the animals exposed to “Shake+Tea” association have changes in the pattern of behavior, probably by the effect of caffeine present in products. Moreover, was observed an increase of 175% of AST (p<0.05) in animals treated with the association compared to the Control group beyond the ~10 fold increase of AOPP in the renal tissue of all the groups treated with oral supplementation (p<0.05). Histological changes were not observed. Conclusion: According to behavioral and biochemical evaluations, we suggest the need for further experimental studies with non herbal and herbal products to state reasons for the safety of these substances widely consumed by the population.